Elevated UGT8 expression highly correlates with BLBC. (A) Box plots indicated UGT8 mRNA expression in different subtypes of breast cancer from four gene expression datasets (NKI295, METABRIC, GSE25066, and TCGA). Comparisons were analyzed by one-way ANOVA. (B) Box plot indicated UGT8 protein expression in different subtypes of breast cancer from TCGA dataset. Comparisons were analyzed by one-way ANOVA. (C) Expression of UGT8 and sulfatide was examined in tumor samples from five cases of luminal and five cases of TNBC. (D) Box plots indicated UGT8 mRNA expression in luminal and BLBC cell lines from four gene expression datasets (GSE12777, GSE10890, E-TABM-157, and E-MTAB-181). Comparisons were made using the two-tailed Student’s t test. (E and F) Expression of UGT8 mRNA was analyzed by either semi-quantitative RT-PCR (E) or quantitative RT-PCR (F) in a representative panel of breast cancer cell lines. Data are shown as mean ± SD based on three independent experiments. *, P < 0.05 by Student’s t test. (G) Expression of UGT8 in cells from E was examined by Western blotting.