Macrophage production of inflammatory mediators is not obviously affected by Shp2 deficiency. (A–C) Peritoneal macrophages (PMs) from Shp2^M WT and Shp2^M KO mice were stimulated with LPS (100 ng/ml; A), PGN (2 µg/ml; B), or heat-killed E. coli (multiplicity of infection = 0.1; C), the expression of TNF-α, IL-6, and IL-1β was determined by qPCR. (D–F) PMs were stimulated with LPS (100 ng/ml), surface expression of CD80, CD86, and MHC-II (D), or intracellular production of ROS (E) or iNOS (F) were detected by flow cytometry. Data are mean ± SEM and are representative of three independent experiments. ***, P < 0.001; two-tailed unpaired Student’s t test.