Shp2 deficiency in macrophages inhibits colitis-associated colon cancer. (A) Experimental design of AOM/DSS-induced colon cancer. (B) Colonic tumors were photographed (left) and counted (right). n = 9–11. Data are mean ± SEM and are compiled from two independent experiments. (C) Representative H&E staining of colon tissues. Bar, 100 µm. (D) The frequencies of different cell populations in CLP were analyzed by flow cytometry. Representative plots showed the myeloid populations. (E) The expression of indicated cytokines in CLP was determined by qPCR. Data are mean ± SEM and are representative of two independent experiments. *, P < 0.05; **, P < 0.01; two-tailed unpaired Student’s t test.