Figure 8. Exogenous SCFA propionate confers protection against severe bronchiolitis and subsequent asthma in pDC-depleted neonatal mice through a Sema4a-dependent mechanism. (a) Nrp-1+ T reg cells in lung and MLN. (b) Airway epithelium sloughing. (c and d) Neutrophil (c) and eosinophil (d) numbers in BAL. (e) Type-2 ILCs in the lung. (f) ASM area. (g) Intraluminal mucus plugging. (h) Eosinophil number in BAL. (i) AHR. Data are representative of n = 2 experiments with four to six neonates in each group and presented as the mean ± SEM (i) or box-and-whisker plots showing quartiles (boxes) and range (whiskers; a–h). Data were analyzed by one-way (a–h) or two-way (i) ANOVA with Tukey’s post hoc test; *, P < 0.05; **, P < 0.01 compared with vehicle-treated WT mice; #, P < 0.05; ##, P < 0.01; ###, P < 0.001 compared with vehicle-treated pDCΔ mice. See also Fig. S3.
Figure 8.

Exogenous SCFA propionate confers protection against severe bronchiolitis and subsequent asthma in pDC-depleted neonatal mice through a Sema4a-dependent mechanism. (a) Nrp-1+ T reg cells in lung and MLN. (b) Airway epithelium sloughing. (c and d) Neutrophil (c) and eosinophil (d) numbers in BAL. (e) Type-2 ILCs in the lung. (f) ASM area. (g) Intraluminal mucus plugging. (h) Eosinophil number in BAL. (i) AHR. Data are representative of n = 2 experiments with four to six neonates in each group and presented as the mean ± SEM (i) or box-and-whisker plots showing quartiles (boxes) and range (whiskers; a–h). Data were analyzed by one-way (a–h) or two-way (i) ANOVA with Tukey’s post hoc test; *, P < 0.05; **, P < 0.01 compared with vehicle-treated WT mice; #, P < 0.05; ##, P < 0.01; ###, P < 0.001 compared with vehicle-treated pDCΔ mice. See also Fig. S3.

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