Figure 4. pDC expression of Sema4a expands Nrp-1+ T reg cells during early-life PVM infection. (a) Protocol for APC and CD4+ T cell co-culture. (b and e) Mean fluorescence intensity of CFSE in Nrp1+ T reg cells. (c) Sema4a expression on MLN APCs at 7 dpi. (d) Sema4a expression on MLN pDCs. For panels b and e, each data point represents a technical replicate and is representative of n = 2 independent experiments. For panels c and d, data are representative of n = 2 independent experiments with four to six neonates in each group and are presented as box-and-whisker plots showing quartiles (boxes) and range (whiskers). Data were analyzed by one-way ANOVA with Tukey post hoc test (b, c, and e) or Mann–Whitney U test (d); #, P < 0.05; **, P < 0.01; ***, P < 0.001 compared with unstimulated cells (b and e), pDCs (c), naive group (d), or as indicated.
Figure 4.

pDC expression of Sema4a expands Nrp-1+ T reg cells during early-life PVM infection. (a) Protocol for APC and CD4+ T cell co-culture. (b and e) Mean fluorescence intensity of CFSE in Nrp1+ T reg cells. (c) Sema4a expression on MLN APCs at 7 dpi. (d) Sema4a expression on MLN pDCs. For panels b and e, each data point represents a technical replicate and is representative of n = 2 independent experiments. For panels c and d, data are representative of n = 2 independent experiments with four to six neonates in each group and are presented as box-and-whisker plots showing quartiles (boxes) and range (whiskers). Data were analyzed by one-way ANOVA with Tukey post hoc test (b, c, and e) or Mann–Whitney U test (d); #, P < 0.05; **, P < 0.01; ***, P < 0.001 compared with unstimulated cells (b and e), pDCs (c), naive group (d), or as indicated.

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