Transcriptional and epigenetic alterations in c-Kit⁺ KI cells. (A) GSEA of c-Kit+ cells showing gene sets positively or negatively associated with expression of mutant Asxl1. KI, Vav-Cre–positive Asxl1-MT^fl/fl; control, Vav-Cre–negative Asxl1-MT^fl/fl. (B) Overlap of up-regulated (top) and down-regulated (bottom) genes both in KI (blue) and KO (red) cells. (C) GO biological process enrichment analysis of up-regulated (top) and down-regulated (bottom) genes only in KI cells. (D) Anti-H2AK119Ub, H3K4me3, H3K27me3, and H3 Western blot in BM cells of control and KI mice. (E) Heatmap representation of H3K4me3 (left) and H2AK119Ub (right). ChIP-seq signals centered on TSSs. (F) RNA-seq and H3K4me3, H3K27me3, and H2AK119Ub ChIP-seq sequencing reads across the Hoxa, Id3, Tjp1 loci in control (blue) and KI c-Kit⁺ (red) cells. (G) Quantitative RT-PCR results of the indicated genes. Control (Vav-Cre–negative Asxl1-MT^fl/fl) and KI (Vav-Cre–positive Asxl1-MT^fl/fl) c-Kit⁺ BM cells (n = 4 each) were used. (H) Absolute number of CFU-E and BFU-E colonies after adding back Id3 into control (Vav-Cre–negative Asxl1-MT^fl/fl) and KI (Vav-Cre–positive Asxl1-MT^fl/fl) BM cells. Data are presented as mean ± SEM if not otherwise specified. **, P < 0.01; ***, P < 0.001; ****, P < 0.0001 (Student’s t test). NES, normalized enrichment score.