Figure 7. SI Mfs have attenuated... | Rockefeller University Press

$Figure 7. SI Mfs have attenuated cytokine production. (A) Spontaneous cytokine release by sorted SI Mf subsets and DCs (n = 8) and CD14+ PBMos and PBMo-lib (n = 7) was measured by a Bio-Plex assay in supernatants after 21 h of culture. Bars indicate median values. #, P < 0.05; ##, P < 0.01; ###, P < 0.001; ####, P < 0.0001 (ANOVA of PBMo, PBMo-lib, and Mf1). **, P < 0.01; ***, P < 0.001; ****, P < 0.0001 (RM-ANOVA of SI subsets). (B) Concentration of cytokines released spontaneously (dots) and after stimulation with 1 µg/ml LPS (squares) in supernatants after 21 h of culture of sorted SI Mf subsets and DCs (n = 5). *, P < 0.05; **, P < 0.01; ***, P < 0.001; ****, P < 0.0001 (two-way RM-ANOVA). (C) Concentration of cytokines released spontaneously (dots) and after stimulation with 1 µg/ml LPS (squares) in supernatants after 21 h of culture of sorted CD14+ PBMos and PBMo-lib (n = 6 or 7). *, P < 0.05; **, P < 0.01; ***, P < 0.001; ****, P < 0.0001 (two-way RM-ANOVA). (D) The percentage of TNFα+ cells in SI Mf subsets was determined by intracellular staining and flow cytometric analysis after 4 h of culture in medium only or containing LPS, flagellin, R848, or poly(I:C) (n = 3). Floating bars represent range, with a line indicating median. Dotted line represents background staining on Mf3 and Mf4 based on the fraction of cells found within the isotype control gate. *, P < 0.05; **, P < 0.01 compared with matched medium-only control (two-way RM-ANOVA).$

Figure 7.

SI Mfs have attenuated cytokine production. (A) Spontaneous cytokine release by sorted SI Mf subsets and DCs (n = 8) and CD14⁺ PBMos and PBMo-lib (n = 7) was measured by a Bio-Plex assay in supernatants after 21 h of culture. Bars indicate median values. ^#, P < 0.05; ^##, P < 0.01; ^###, P < 0.001; ^####, P < 0.0001 (ANOVA of PBMo, PBMo-lib, and Mf1). **, P < 0.01; ***, P < 0.001; ****, P < 0.0001 (RM-ANOVA of SI subsets). (B) Concentration of cytokines released spontaneously (dots) and after stimulation with 1 µg/ml LPS (squares) in supernatants after 21 h of culture of sorted SI Mf subsets and DCs (n = 5). *, P < 0.05; **, P < 0.01; ***, P < 0.001; ****, P < 0.0001 (two-way RM-ANOVA). (C) Concentration of cytokines released spontaneously (dots) and after stimulation with 1 µg/ml LPS (squares) in supernatants after 21 h of culture of sorted CD14⁺ PBMos and PBMo-lib (n = 6 or 7). *, P < 0.05; **, P < 0.01; ***, P < 0.001; ****, P < 0.0001 (two-way RM-ANOVA). (D) The percentage of TNFα⁺ cells in SI Mf subsets was determined by intracellular staining and flow cytometric analysis after 4 h of culture in medium only or containing LPS, flagellin, R848, or poly(I:C) (n = 3). Floating bars represent range, with a line indicating median. Dotted line represents background staining on Mf3 and Mf4 based on the fraction of cells found within the isotype control gate. *, P < 0.05; **, P < 0.01 compared with matched medium-only control (two-way RM-ANOVA).

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