SI Mf subsets are distinguished by their gene expression patterns. (A) PCA of top 1,000 differentially expressed genes within CD14⁺ PBMo, PBMo-lib (enzyme-treated CD14⁺ PBMos), SI Mf, and DC subsets and in vitro differentiated Mfs. (B) Pearson correlation and hierarchical clustering analysis of CD14⁺ PBMo, PBMo-lib, SI Mf, and DC subsets and in vitro Mfs. Fig. S4 D lists all correlation values. (C) Polytomous analysis of differentially expressed genes among CD14⁺ PBMo and SI Mf and DC subsets, grouped into 16 clusters based on top models obtained from unsupervised clustering. Genes were assigned to a model based on the highest calculated posterior probability value (>0.65). Expression values are log2FPKM. Top-ranked genes from each cluster are listed on the right (full list available in Table S1). (A–C) CD14⁺ PBMos and SI Mfs were isolated from five donors, SI DC subsets from two of these. PBMo-lib were derived from two of the included CD14⁺ PBMo samples. In vitro Mfs were differentiated from PBMos of two separate healthy donors.