Macrophage-restricted IL-10 deletion in SAUNA-exposed mice improves diastolic function. (A) Experimental outline of the SAUNA protocol applied on mice lacking IL-10. (B) Flow cytometric quantification of neutrophils and macrophages in hearts from littermate and Cx₃cr1 Il10^−/− SAUNA-exposed mice. Left: Representative flow cytometry plots; right: number of neutrophils and macrophages per milligram of heart tissue. The pie charts indicate the percentage of MHCII^low (orange) and MHCII^high (purple) cardiac macrophages. Data are pooled from two independent experiments (n = 14–16 mice per group). (C) Immunohistochemical analysis of macrophages in hearts from littermate and Cx₃cr1 Il10^−/− SAUNA-exposed mice. Left: Representative images; right: bar graph shows percentage of positive staining per ROI. Data are pooled from two independent experiments (n = 6–7 mice per group). Bar, 25 µm. (D) Hemodynamic parameters by pressure–volume catherization of hearts from littermate and Cx₃cr1 Il10^−/− SAUNA-exposed mice. Data are pooled from two independent experiments (n = 7–11 mice per group). (E) Serum creatinine levels in littermate and Cx₃cr1 Il10^−/− SAUNA-exposed mice. Data are pooled from two independent experiments (n = 6 mice per group). (F) Systolic blood pressure in littermate and Cx₃cr1 Il10^−/− SAUNA-exposed mice. Data are pooled from six independent experiments (n = 38–46 mice per group). (G) Relative Anp and Bnp expression levels by qPCR in hearts from littermate and Cx₃cr1 Il10^−/− SAUNA-exposed mice. Data are pooled from four independent experiments (n = 27 mice per group). (H) Lung wet-to-dry weight ratio in littermate and Cx₃cr1 Il10^−/− SAUNA-exposed mice. Data are pooled from five independent experiments (n = 32–39 mice per group). Results are shown as mean ± SD. For statistical analysis, a two-tailed unpaired t test was performed to compare two groups. *, P < 0.05; **, P < 0.01; ***, P < 0.001.