Heterogeneity of early MyPs revealed by scRNA-Seq. Shown is scRNA-Seq analysis of Lin⁻ Tom⁺ cells 2 wk after HSC labeling, focusing on the myeloid cell cluster (cluster 0 in Fig. 5). (A) Violin plots of the indicated myeloid genes expression in the MyP cluster. (B–G) tSNE plots (arbitrary units) of the isolated MyP cluster shown as in Fig. 5 A. The range of color scale of log₁₀ normalized gene counts is indicated for each feature plot. (B) Feature plots of the indicated myeloid cell type–specific genes. (C) Feature plots of the indicated myeloid TFs. (D) Proposed lineage assignments of cells in the myeloid cell cluster. (E) Feature plots of Irf8 and Gfi1 expression, with cells coexpressing both genes highlighted in blue circles. (F) Feature plots of dendritic cell lineage–specific marker genes and TFs. (G) Feature plots of Tcf4 and Id2 expression in the myeloid cell cluster, with cells coexpressing both genes highlighted in blue circles.