Figure 6. Heterogeneity of early EryPs revealed by scRNA-Seq. Shown is scRNA-Seq analysis of Lin− Tom+ cells 2 wk after HSC labeling, focusing on the erythroid cell cluster (cluster 3 in Fig. 5). (A) Violin plots of the indicated erythroid genes expression in the EryP cluster. (B–E) tSNE plots (arbitrary units) of the isolated EryP cluster shown as in Fig. 5 A, highlighting the two subclusters (B) and the expression of indicated TFs (C), lineage marker genes (D), and proliferation signature genes (E). The range of color scale of log10 normalized gene counts is indicated for each feature plot.
Figure 6.

Heterogeneity of early EryPs revealed by scRNA-Seq. Shown is scRNA-Seq analysis of Lin− Tom+ cells 2 wk after HSC labeling, focusing on the erythroid cell cluster (cluster 3 in Fig. 5). (A) Violin plots of the indicated erythroid genes expression in the EryP cluster. (B–E) tSNE plots (arbitrary units) of the isolated EryP cluster shown as in Fig. 5 A, highlighting the two subclusters (B) and the expression of indicated TFs (C), lineage marker genes (D), and proliferation signature genes (E). The range of color scale of log10 normalized gene counts is indicated for each feature plot.

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