The analysis of differentiating HSC progeny at 2 wk by scRNA-Seq. Adult Pdzk1ip1-CreER R26^tdTomato reporter mice were given a single dose of tamoxifen, and Lin⁻ Tom⁺ cells from the BM were isolated 2 wk later and profiled by scRNA-Seq. Cells were pooled from two independent experiments (total of three individual animals). (A) Unsupervised clustering of all pooled cells. Shown is tSNE plot (arbitrary units) of individual cells colored by the cluster (Cl.). (B) Top 10 differentially expressed genes within each cluster in panel A. Shown is a heat map with the level of indicated genes (rows) in individual cells (columns) expressed on a color scale representing z-score distribution. (C) Feature plots showing the expression of the indicated marker genes in individual cells on the same tSNE plot as in A. Shown are genes characteristic of undifferentiated HSCs (Mllt3 and Sult1a1); differentiating progenitors (Plac8 and Mpo); and megakaryocytic (Pf4 and Plek), myeloid (Ctsg and Elane), erythroid (Car1 and Ermap), and lymphoid (Dntt and Rag2) differentiation. The range of color scale of log₁₀ normalized gene counts is indicated for each feature plot.