Sox7 expression in tumor vessels correlates with poor prognosis and impaired tumor vessels of grade IV glioma patients. (A) Categorization of grade IV glioma patients based on Sox7 levels in tumor vessels. (B) Immunostaining for Sox7 and Sox17. (C and D) Overall survival (C) and progression-free survival (D) of Sox7-positive (n = 62) and Sox7-negative (n = 127) patients. *, P < 0.05; **, P < 0.01 (log-rank test). (E–L) Representative images of Sox7-negative (E–H) and Sox7-positive (I–L) patients. (E and I) H&E images (left). Enlarged images of boxed area (right). (F and J) DCE MR images (left) and enlarged views of red-lined box (right). (G and K) Perfusion MR images (left) and enlarged views of red lined box (right). Insets are regions for measuring flow decay. Color index denotes the intensity of blood flow signal. Red dotted lines: tumor margin. Blue dotted lines: peritumoral edema margin. Asterisk denotes copious peritumoral leakage. (H and L) Negative integral map showing blood flow decay at multiple regions. Each line denotes the dynamic flow signal from single inset in G and K (right). Delayed signal recovery indicates vessel leakage caused by poor removal of extravasated gadolinium. See Fig. S4 (F and G) for quantitation of intratumoral leakage (F, H, J, and L) and tumor perfusion (G and K), respectively. Bars: 100 µm (B, E [right], and I [right]); 200 µm (E, left; I, left).