Decreased tubular NLRX1 expression in ATN and ACR biopsies. (A) Representative example of NLRX1 immunostaining performed on a protocol biopsy at 6 mo after renal transplantation. Expression of NLRX1 by (B) renal vascular smooth muscle cells. Absent or low NLRX1 labeling in biopsies diagnosed with (C) ATN and (D) ACR. (E) NLRX1 expression by inflammatory cells but not tubular epithelium in ACR. (F) Semiquantitative scoring of tubular staining intensity for NLRX1. Data are expressed as mean ± SEM and were analyzed using a Mann-Whitney U test (*, P < 0.05 vs. both controls). Pre-Tx, pretransplantation kidney (n = 3); prot Bx, protocol biopsy at 6 mo after transplantation (n = 4); ATN (n = 11) and ACR (n = 10). Bars, 50 µm.