Impact of Cxcr4¹⁰¹³-bearing nonhematopoietic cells on hematopoiesis. (A) Schematic diagram for the generation of long-term BM chimeras. (B) Absolute numbers of donor CD45.1⁺ (WT) total CD3⁺ T and B220⁺ B cells, pro–B cells, and ETPs recovered from the blood, BM, and thymus of BM chimeras in CD45.2⁺ (WT, +/1013 or 1013/1013) recipients. Data are from at least three independent experiments with 6–10 recipient mice per group. (C) Absolute numbers of donor CD45.1⁺ (Gr1⁺CD11b⁺) myeloid cells recovered from the blood and BM of BM chimeras in CD45.2⁺ recipients. Data are from at least two independent experiments with four to nine recipient mice per group. (D) Proportions (left) and absolute numbers (middle and right) of donor CD45.1⁺ LSK and LSK SLAM recovered from the BM of chimeras in CD45.2⁺ recipients. Data are from at least three independent experiments with 6–15 recipient mice per group. (E) Proportions (left) and absolute numbers (middle and right) of donor CD45.1⁺ LSK SLAM, CMPs, GMPs, and MEPs recovered from the spleens of chimeras in CD45.2⁺ recipients. Data are from at least three independent experiments with 6–15 recipient mice per group. All displayed results are represented as means ± SEM. Kruskal–Wallis H test–associated p-values (#) are indicated. **, P < 0.005; and ^§§, P < 0.005 compared with WT and +/1013 recipients, respectively (as determined using the two-tailed Student’s t test).