Changes in gene expression in Apc^Min/+::ADAM17^ex/ex mice. (A) Laser microdissection and qRT-PCR analysis of ErbB family receptors and ligands from dysplasia of Apc^Min/+ mice and Apc^Min/+::ADAM17^ex/ex mice. Tissue samples from six dysplasias from Apc^Min/+ mice (n = 3) and four dysplasias from Apc^Min/+::ADAM17^ex/ex mice (n = 2) were collected via laser microdissection and compared with WT tissue. qRT-PCR analysis was performed in triplicate. ***, P < 0.001 by two-way ANOVA with Bonferroni post hoc test. (B) qRT-PCR analysis of selected target genes from Apc^Min/+ mice and Apc^Min/+::ADAM17^ex/ex mouse tissue. 26 tumor samples obtained from Apc^Min/+ mice (n = 7) and 10 tumor samples obtained from Apc^Min/+::ADAM17^ex/ex mice (n = 4) were compared with tissue samples from WT mice (n = 1). *, P < 0.05; **, P < 0.01; ***, P < 0.001; ns, nonsignificant by unpaired t test with Welch’s correction. Error bars indicate means ± SD.