CBF-β and TOX are required before the EILP stage. (A–C) Flow cytometric analysis of bone marrow cells from Vav1-iCre Cbfb^f/f mice and Vav1-iCre Cbfb^+/+ littermate controls. Profiles of ALPs (A) and EILPs (B) are shown after gating on Lin⁻ Kit⁺ CD122^low cells. (C) Quantification of ALP, EILP, ILCp, B cell, and granulocyte numbers. (D and E) Flow cytometric analysis of bone marrow cells from Tox^−/− mice and Tox^+/+ littermate controls. (D) Profiles of EILPs are shown after gating on Lin⁻ Kit⁺ CD122^low cells. (E) Quantification of ALP, EILP, and ILCp numbers. Numbers indicate the percentage of cells in each gate. All data are representative of at least two independent experiments and are presented as mean ± SEM for n = 3 mice per group. A two-tailed unpaired Student’s t test was performed to determine significance. *, P < 0.05; **, P < 0.01; ***, P < 0.005.