Figure 3. The conduit system transports soluble IgM upon intranodal injection. (A and B) JHT mice were injected intradermally with CFA at the base of the tail. 10 d later, draining inguinal LNs were surgically exposed and microinjected with fluorescent WGA with or without purified mouse IgM (A) or 150-kD fluorescent dextran (DEX; B). Data show confocal images from inguinal LN sections stained for collagen IV (Col.IV) and IgM. Insets display high-magnification views of conduits from the diffusion front. Bars, 100 µm (left panels); 10 µm (right panels). Data are representative of three experiments (two mice per condition and experiment) in A and B.
Figure 3.

The conduit system transports soluble IgM upon intranodal injection. (A and B) JHT mice were injected intradermally with CFA at the base of the tail. 10 d later, draining inguinal LNs were surgically exposed and microinjected with fluorescent WGA with or without purified mouse IgM (A) or 150-kD fluorescent dextran (DEX; B). Data show confocal images from inguinal LN sections stained for collagen IV (Col.IV) and IgM. Insets display high-magnification views of conduits from the diffusion front. Bars, 100 µm (left panels); 10 µm (right panels). Data are representative of three experiments (two mice per condition and experiment) in A and B.

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