B cell development in the Shh^fl/flVavCre FL. (A–H) Flow cytometry of the E14.5 FL (A–C), E16.5 FL (D and E), and E18.5 FL (F–H) from Shh^fl/flVavCre⁻ (E14.5, n = 5; E16.5, n = 3; E18.5, n = 5) and Shh^fl/flVavCre⁺ (E14.5, n = 3; E16.5, n = 5; and E18.5, n = 3) littermates. (A, D, and F) Dot plots: SSC against CD19 staining (left), CD19 staining against B220 staining (middle), and µH staining for D and F (right). (B, E, and G) Bar charts: mean percentage, relative to mean of WT littermates ± SEM of the B220⁺, CD19⁺, CD19⁺B220⁺, CD19⁻B220⁺, CD19⁺B220⁻, CD19⁺BP-1⁺, and CD19⁺µH⁺ populations. (C and H) Bar charts: mean percentage ± SEM of Lin⁻CD117⁺CD127⁺ CLPs. There were no significant differences by Student’s t test. Shaded bars are Cre⁺, and unshaded bars are Cre⁻.