RIC induces anti-inflammatory macrophage polarization after ICH. (A and B) Mixed sex C57BL/6J littermates were randomized to receive once-daily mock conditioning or bilateral RIC beginning at 2 h after sham or collagenase-induced ICH. Phenotypic analysis of macrophages was performed in peri-hematoma brain tissue (0.2 g) or blood (B; 200 µl) at 72 h after sham, ICH with mock conditioning (ICH), or ICH+RIC. Live cells were gated using FSC/SSC and CD11b⁺F4/80⁺ cells were further selected. CD11b⁺CD45^hi-infiltrated myeloid cells and CD11b⁺CD45^low residential myeloid cells were selected from brain tissue, or CD11b⁺CD68⁺ macrophages were selected from blood. Selected populations were further defined using F4/80, CD36, MerTK, MHC-II, CD206, Ly-6C, Ly-6G, or IL-10. Representative histograms are provided for each marker. Gray shaded areas indicate isotype controls. Quantified data, which are expressed as the mean ± SEM from n = 6 mice/group, were analyzed using a one-way ANOVA followed by Tukey’s post-hoc test (*, P < 0.05; **, P < 0.01; ***, P < 0.001; ****, P < 0.0001; ns, not statistically significant) and are representative of two independent experiments.