Group 1 Vγ9Vδ2 T cells are functionally more potent in their response to type I EBV infection than group 2 Vγ9Vδ2 T cells. PBMCs from six group 1 donors and six group 2 donors were incubated in culture either alone (Ø) or in the presence of target cells. The targets were Raji (a negative control), Akata, and Daudi. (A) Boxes and whiskers represent the proportion of granzyme B⁺ Vγ9Vδ2 T cells from group 1 donors (orange) and group 2 donors (green) after 24-h culture. (B) Boxes and whiskers represent the proportion of IFNγ⁺ Vγ9Vδ2 T cells from group 1 donors (orange) and group 2 donors (green) after 24-h culture. (C) Boxes and whiskers represent the proportion of MIP1-β⁺ Vγ9Vδ2 T cells from group 1 donors (orange) and group 2 donors (green) after 24-h culture. Statistical significance of the difference between experiment and control was assessed using ANOVA (*, P ≤ 0.04; **, P < 0.004; ***, P ≤ 0.0006; ns, nonsignificant).