In NS patients, serum TSLP levels positively correlate with CXCL13. (A) FACS analysis showing the frequency of CCR6⁻CXCR3⁻ (green), CCR6⁻CXCR3⁺, and CCR6⁺CXCR3⁻ populations in the CD4⁺CD45RO⁺CXCR5⁺ gate. Representative plots are shown for a healthy donor and AD donor, respectively. Frequency distribution in six AD donors, and four healthy donors are plotted. *, P < 0.05; **, P < 0.01; ***, P < 0.001, paired Student’s t test. (B) Linear correlation between serum TSLP and CXCL13, measured by ELISA, is shown. Spearman r and P-values are plotted. 64 samples from 13 NS patients are plotted.