TSLP-induced cells express Tfh markers. FACS analysis of BTLA (A), CD200 (B), SAP (C), C-MAF (D), and CCR7 (E) in naive CD4 T cells and in TSLP-DC and LPS-DC activated cells at day 4 of co-culture with naive CD4 T cells. Isotype and specific staining for naive CD4 T cells and CXCR5^hi/PD1^hi and CXCR5^lo/PD1^lo population induced by TSLP-DC and LPS-DC is shown in histogram plot for one representative experiment. Quantification of MFI is plotted for three or four independent experiments. Naive CD4 T cells, filled diamond; TSLP-DC co-culture, filled triangles; LPS-DC co-culture, triangles. *, P < 0.05; **, P < 0.01; ***, P < 0.001, paired Student’s t test.