T cells polarized by TSLP-DC show an expression pattern similar to tonsillar Tfh cells. Heat map showing mRNA quantification of Tfh and Th markers in naive CD4 T cells, TSLP-DC–polarized CD4 T cells, and human tonsillar CD4 populations. CD4 T cells differentiated for 4 d with TSLP-DC were sorted as indicated (top left). Three populations of tonsillar CD4 cells were sorted (top right): CXCR5^hi/PD1^hi (GC Tfh), CXCR5^int/PD1^int (Tfh), and CXCR5⁻/PD1⁻. mRNA levels normalized on the B2M and RPL34 housekeeping genes and center reduced are displayed on the heat map from five independent donors and two independent experiments.