Figure 2. Leukocyte extravasation in the posthypoxic Anxa2−/− lung. (A) Representative images of hematoxylin and eosin–stained sections from Anxa2+/+ and Anxa2−/− normoxic or posthypoxic lungs. Bars, 20 µm. (B and C) Quantification of inflammatory cell infiltration and septal thickness in 20 random sections from each of three highly perfused lungs from Anxa2+/+ or Anxa2−/− normoxic (B) or posthypoxic (C) mice (ANOVA). (D) Immunoblot of lung homogenates from three separate animals for neutrophil (Ly6G) and macrophage (F4/80) markers. Results in A–E are representative of three separate experiments. (E) Quantification of immunoblotted Ly6G/GAPDH and F4/80/GAPDH ratios from (D; n = 3 mice per group, ANOVA). Black and white bars indicate Anxa2+/+ and Anxa2−/− mice, respectively. Data are expressed as mean ± SEM. **, P < 0.01; ***, P < 0.001.
Figure 2.

Leukocyte extravasation in the posthypoxic Anxa2−/− lung. (A) Representative images of hematoxylin and eosin–stained sections from Anxa2+/+ and Anxa2−/− normoxic or posthypoxic lungs. Bars, 20 µm. (B and C) Quantification of inflammatory cell infiltration and septal thickness in 20 random sections from each of three highly perfused lungs from Anxa2+/+ or Anxa2−/− normoxic (B) or posthypoxic (C) mice (ANOVA). (D) Immunoblot of lung homogenates from three separate animals for neutrophil (Ly6G) and macrophage (F4/80) markers. Results in A–E are representative of three separate experiments. (E) Quantification of immunoblotted Ly6G/GAPDH and F4/80/GAPDH ratios from (D; n = 3 mice per group, ANOVA). Black and white bars indicate Anxa2+/+ and Anxa2−/− mice, respectively. Data are expressed as mean ± SEM. **, P < 0.01; ***, P < 0.001.

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