Figure 1. Efficiency of sgRNAs flanking the mouse JH region. (a) Example chromatograms obtained by blastocyst PCR, 4 d after CRISPR-Cas9–mediated targeting by zygote injection. WT (protospacer and PAM indicated; top) and successfully targeted blastocysts (bottom). Note the altered peaks resulting from a monoallelic indel at the position indicated with an arrowhead (repair site). (b) List of tested sgRNA protospacer sequences, including mouse strain, location (5′ or 3′ of the J segments), and efficiency of cutting measured as in panel a. The final sgRNAs used for generating knock-in mice are in bold font.
Figure 1.

Efficiency of sgRNAs flanking the mouse JH region. (a) Example chromatograms obtained by blastocyst PCR, 4 d after CRISPR-Cas9–mediated targeting by zygote injection. WT (protospacer and PAM indicated; top) and successfully targeted blastocysts (bottom). Note the altered peaks resulting from a monoallelic indel at the position indicated with an arrowhead (repair site). (b) List of tested sgRNA protospacer sequences, including mouse strain, location (5′ or 3′ of the J segments), and efficiency of cutting measured as in panel a. The final sgRNAs used for generating knock-in mice are in bold font.

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