Figure 4.
Figure 4. mTECs express the type 2 IL-4R. (A) Quantitative PCR of cells from adult WT mouse thymus and spleen, error bars indicate SEM, and data are typical of three independent experiments. (B) Quantitative PCR of sorted EpCAM1+ TECs from WT 2dGuo FTOC ± IL-4/IL-13 for 4 d. mRNA levels were normalized to β-actin; error bars indicate SEM, and data are typical of three independent experiments. (C) Untreated and IL-4/IL-13–stimulated EpCAM1+ TEC from 2dGuo FTOC stained with anti-phospho-STAT6, n = 4 from three independent experiments. (D) Confocal images of WT and Il4ra−/− mice 2dGuo FTOC 10-wk-old thymus transplants; data represent at least two experiments, n = 6 for WT and Il4ra−/− mice. Bars, 50 μm. (E) Analysis of conventional SP4 thymocytes from WT and Il4ra−/− thymic grafts; data represent at least two experiments, and n = 6 for WT and Il4ra−/− mice. Error bars indicate SEM. A Mann-Whitney nonparametric U test was performed. *, P < 0.05.

mTECs express the type 2 IL-4R. (A) Quantitative PCR of cells from adult WT mouse thymus and spleen, error bars indicate SEM, and data are typical of three independent experiments. (B) Quantitative PCR of sorted EpCAM1+ TECs from WT 2dGuo FTOC ± IL-4/IL-13 for 4 d. mRNA levels were normalized to β-actin; error bars indicate SEM, and data are typical of three independent experiments. (C) Untreated and IL-4/IL-13–stimulated EpCAM1+ TEC from 2dGuo FTOC stained with anti-phospho-STAT6, n = 4 from three independent experiments. (D) Confocal images of WT and Il4ra−/− mice 2dGuo FTOC 10-wk-old thymus transplants; data represent at least two experiments, n = 6 for WT and Il4ra−/− mice. Bars, 50 μm. (E) Analysis of conventional SP4 thymocytes from WT and Il4ra−/− thymic grafts; data represent at least two experiments, and n = 6 for WT and Il4ra−/− mice. Error bars indicate SEM. A Mann-Whitney nonparametric U test was performed. *, P < 0.05.

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