IL-4Rα regulates thymic egress. (A and B) TCRβ⁺PBS57⁻CD44⁻Foxp3⁻ SP4 T-conv thymocytes from WT and Il4ra^−/− mice. (C and D) Rag2GFP levels in indicated SP4 thymocyte subsets from WT mice. (E and F) SP4 T-conv quantitation in WT and Il4ra^−/− mice. (G) Rag2GFP in SP4 T-conv from WT and Il4ra^−/− mice and frequency of GFP⁻ cells in WT and Il4ra^−/− mice. (H) Rag2GFP in conventional SP4 thymocytes from WT (blue) and Il4ra^−/− (red) mice with mean fluorescence intensity (MFI) of Rag2GFP. (I) Splenic CD4⁺Foxp3⁻ T cells in WT and Il4ra^−/− mice. (J) Frequency, number, and Rag2GFP levels in splenic WT and Il4ra^−/− GFP⁺ RTE. All data represent at least three experiments, n = 10 for both WT and Il4ra^−/− mice. Error bars indicate SEM. A Mann-Whitney nonparametric U test was performed; *, P < 0.05; **, P < 0.01; ***, P < 0.001; ****, P < 0.0001.