Figure 5. FBXL14 is preferentially expressed in nonstem glioma cells and NPCs. (A) IB analysis of FBXL14, c-Myc, OLIG2, and GFAP in GSCs (+) and matched NSTCs (−) isolated from five GBM tumors. OLIG2 and c-Myc are GSC markers, whereas GFAP is a marker for differentiated cells (astrocytes). FBXL14 is preferentially expressed in NSTCs. (B) Immunofluorescent staining of FBXL14 (green) and c-Myc (red) in GSCs and matched NSTCs derived from T387 xenografts. Nuclei were counterstained with DAPI (blue). FBXL14 is expressed in NSTCs but rarely expressed in the GSCs. (C) Immunofluorescent staining of FBXL14 (green) and the differentiation marker (GFAP; red) in GSCs and matched NSTCs derived from T387 xenografts. Nuclei were counterstained with DAPI (blue). FBXL14 is preferentially expressed in the NSTCs. (D) IB analysis of FBXL14, the GSC markers (c-Myc and SOX2), and the differentiated cell markers (GFAP, TUJ1, and MAP2) during serum-induced GSC differentiation. T4121 GSCs were cultured in DMEM containing 10% FBS to induce differentiation and harvested on the indicated days for immunoblotting. c-Myc and SOX2 gradually decreased, whereas FBXL14 along with the differentiation markers GFAP (for astrocytes) and MAP2 (for neurons) increased during GSC differentiation. (E–G) Immunofluorescent staining of FBXL14 and the differentiation marker MAP2 (E) or GFAP (F) or GSC marker SOX2 (G) in a primary GBM (CCF2445). Frozen sections of GBM were coimmunostained with specific antibodies against FBXL14 (green) and a differentiation marker (MAP2 or GFAP; red) or a GSC marker (SOX2; red) and then counterstained with DAPI to show nuclei. FBXL14 is coexpressed in the glioma cells expressing the differentiation markers but not the GSC marker. (H) IB analysis of FBXL14, c-Myc, and SOX2 in GSC populations and human NPC lines. GSCs expressed much less FBXL14 but much more c-Myc than NPCs. (I) Immunofluorescent staining of FBXL14 and SOX2 in human NPCs. NPCs were coimmunostained with specific antibodies against FBXL14 (green) and a stem cell marker (SOX2; red) and then counterstained with DAPI (blue). FBXL14 is expressed in NPCs. (J and K) Immunofluorescent staining of FBXL14 and GFAP, SOX2, or TUJ1 in the dentate gyrus (DG) or SVZ in the adult or embryonic mouse brain. Frozen sections of normal mouse brains were coimmunostained with specific antibodies against FBXL14 (green) and an NPC marker (SOX2; red), an astrocyte marker (GFAP; red), or a neuronal marker (TUJ1; red) and then counterstained with DAPI (blue) to show nuclei. Asterisks indicate the dentate gyrus (J, top) or the SVZ (J, bottom; and K). (A, D, and H) Mass is shown in kilodaltons.
Figure 5.

FBXL14 is preferentially expressed in nonstem glioma cells and NPCs. (A) IB analysis of FBXL14, c-Myc, OLIG2, and GFAP in GSCs (+) and matched NSTCs (−) isolated from five GBM tumors. OLIG2 and c-Myc are GSC markers, whereas GFAP is a marker for differentiated cells (astrocytes). FBXL14 is preferentially expressed in NSTCs. (B) Immunofluorescent staining of FBXL14 (green) and c-Myc (red) in GSCs and matched NSTCs derived from T387 xenografts. Nuclei were counterstained with DAPI (blue). FBXL14 is expressed in NSTCs but rarely expressed in the GSCs. (C) Immunofluorescent staining of FBXL14 (green) and the differentiation marker (GFAP; red) in GSCs and matched NSTCs derived from T387 xenografts. Nuclei were counterstained with DAPI (blue). FBXL14 is preferentially expressed in the NSTCs. (D) IB analysis of FBXL14, the GSC markers (c-Myc and SOX2), and the differentiated cell markers (GFAP, TUJ1, and MAP2) during serum-induced GSC differentiation. T4121 GSCs were cultured in DMEM containing 10% FBS to induce differentiation and harvested on the indicated days for immunoblotting. c-Myc and SOX2 gradually decreased, whereas FBXL14 along with the differentiation markers GFAP (for astrocytes) and MAP2 (for neurons) increased during GSC differentiation. (E–G) Immunofluorescent staining of FBXL14 and the differentiation marker MAP2 (E) or GFAP (F) or GSC marker SOX2 (G) in a primary GBM (CCF2445). Frozen sections of GBM were coimmunostained with specific antibodies against FBXL14 (green) and a differentiation marker (MAP2 or GFAP; red) or a GSC marker (SOX2; red) and then counterstained with DAPI to show nuclei. FBXL14 is coexpressed in the glioma cells expressing the differentiation markers but not the GSC marker. (H) IB analysis of FBXL14, c-Myc, and SOX2 in GSC populations and human NPC lines. GSCs expressed much less FBXL14 but much more c-Myc than NPCs. (I) Immunofluorescent staining of FBXL14 and SOX2 in human NPCs. NPCs were coimmunostained with specific antibodies against FBXL14 (green) and a stem cell marker (SOX2; red) and then counterstained with DAPI (blue). FBXL14 is expressed in NPCs. (J and K) Immunofluorescent staining of FBXL14 and GFAP, SOX2, or TUJ1 in the dentate gyrus (DG) or SVZ in the adult or embryonic mouse brain. Frozen sections of normal mouse brains were coimmunostained with specific antibodies against FBXL14 (green) and an NPC marker (SOX2; red), an astrocyte marker (GFAP; red), or a neuronal marker (TUJ1; red) and then counterstained with DAPI (blue) to show nuclei. Asterisks indicate the dentate gyrus (J, top) or the SVZ (J, bottom; and K). (A, D, and H) Mass is shown in kilodaltons.

This Feature Is Available To Subscribers Only

or Create an Account

Close Modal
Close Modal