The inflammasome mediates LPC-induced IL-1β secretion in microglia. (A and B) ICC of Iba-1 and GFAP staining of mixed glial cell cultures (A) and CD11b-positive selected cells (B) isolated from postnatal day-3–5 WT mouse pups indicates the degree of microglial purity. Bars, 50 µM. (C–G) WT, Nlrc4^−/−, Nlrp3^−/−, Il1b^−/− (C), Asc^−/− (D), Casp-1^−/− (E), Casp-11^−/− (F), and Il18^−/− (G) microglia were LPS primed (100 ng/ml) for 3 h and stimulated with LPC (20, 25, and 50 µM) for 1 h. IL-1β in the cell culture supernatant was assessed by ELISA. Data are representative of two independent experiments. Each experimental condition was performed in triplicate. Results are displayed as the mean ± SEM. *, P < 0.05; **, P < 0.01; ***, P < 0.001; ****, P < 0.0001; unpaired Student's t test.