Figure 2. HK deficiency inhibits LPS-induced cytokine production in mice. (A and B) Kng1−/− mice and their littermate control mice were challenged by PBS (control) or LPS (5 mg/kg) for 4 h, followed by collection of plasma and isolation of PBMCs. Measurements of TNF, IL-1β, IL-6, and HMGB-1 at the protein level in plasma (A) and cytokine mRNA levels in PBMCs (B) were performed as described in Materials and methods. Data were analyzed using an unpaired Student's t test and are representative of two independent experiments. *, P < 0.05; **, P < 0.01. (C) Primary murine monocytes were purified using flow cytometry with PE-labeled antibodies recognizing CD90, B220, CD49b, NK1.1, Ly-6G surface markers, and an APC-labeled anti-CD11b antibody. The purified monocytes were defined as the PE-negative and APC-positive population (dashed line rectangle). The expression of Ly6C, CD115, B220, CD3e, and Ly6G were verified by flow cytometry. (D) Kng1−/− mice and their littermate control mice were challenged with PBS (control) or LPS (5 mg/kg) for 4 h, followed by isolation of monocytes. The IL-1β and IL-6 mRNA levels were measured by quantitative RT-PCR. Data were analyzed using an unpaired Student's t test and are representative of three independent experiments. *, P < 0.05; ***, P < 0.001. Data are expressed as mean ± SEM.
Figure 2.

HK deficiency inhibits LPS-induced cytokine production in mice. (A and B) Kng1−/− mice and their littermate control mice were challenged by PBS (control) or LPS (5 mg/kg) for 4 h, followed by collection of plasma and isolation of PBMCs. Measurements of TNF, IL-1β, IL-6, and HMGB-1 at the protein level in plasma (A) and cytokine mRNA levels in PBMCs (B) were performed as described in Materials and methods. Data were analyzed using an unpaired Student's t test and are representative of two independent experiments. *, P < 0.05; **, P < 0.01. (C) Primary murine monocytes were purified using flow cytometry with PE-labeled antibodies recognizing CD90, B220, CD49b, NK1.1, Ly-6G surface markers, and an APC-labeled anti-CD11b antibody. The purified monocytes were defined as the PE-negative and APC-positive population (dashed line rectangle). The expression of Ly6C, CD115, B220, CD3e, and Ly6G were verified by flow cytometry. (D) Kng1−/− mice and their littermate control mice were challenged with PBS (control) or LPS (5 mg/kg) for 4 h, followed by isolation of monocytes. The IL-1β and IL-6 mRNA levels were measured by quantitative RT-PCR. Data were analyzed using an unpaired Student's t test and are representative of three independent experiments. *, P < 0.05; ***, P < 0.001. Data are expressed as mean ± SEM.

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