Figure 1. Endogenous T reg cells form prolonged contacts with DCs after immunization. (A) Protocol used for visualizing interactions between endogenous T reg cells and OVA-pulsed DCs in the presence or absence of transferred OT-II T cells in vivo. (B and C) Snapshots of interactions between DCs and Foxp3-expressing T reg cells visualized in quiescent (B) or immunized (C) draining LNs where OT-II were being activated by LPS-stimulated, OVA-pulsed DCs (see corresponding Videos 1 and 2). Arrowheads and corresponding numbers trace different T reg cells interacting with the DCs sequentially, covering from the beginning to the end of each T reg cell–DC interaction incidence. Time code, hour:min:sec. Bar, 15 µm. (D) Duration of contacts quantitated for 50 and 52 T reg cell–DC conjugates with or without overt T cell activation. Data are pooled from three independent experiments. ***, P < 0.001, Student’s t test.
Figure 1.

Endogenous T reg cells form prolonged contacts with DCs after immunization. (A) Protocol used for visualizing interactions between endogenous T reg cells and OVA-pulsed DCs in the presence or absence of transferred OT-II T cells in vivo. (B and C) Snapshots of interactions between DCs and Foxp3-expressing T reg cells visualized in quiescent (B) or immunized (C) draining LNs where OT-II were being activated by LPS-stimulated, OVA-pulsed DCs (see corresponding Videos 1 and 2). Arrowheads and corresponding numbers trace different T reg cells interacting with the DCs sequentially, covering from the beginning to the end of each T reg cell–DC interaction incidence. Time code, hour:min:sec. Bar, 15 µm. (D) Duration of contacts quantitated for 50 and 52 T reg cell–DC conjugates with or without overt T cell activation. Data are pooled from three independent experiments. ***, P < 0.001, Student’s t test.

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