Figure 1.
![Figure 1. Image analysis workflow. (A) Overview of image analysis. (B) Preprocessing workflow. (C) Segmentation workflow. (D) Using the collagen signal (second harmonic generation) for classification between autofluorescence artifacts and GFP+RFP+MCs. Red channel refers to MCs, green channel refers to DCs, blue channel shows blood vessels, and gray channel shows collagen. Bars: (A–C and D [right]) 20 µm; (D, left) 50 µm. See also Fig. S1.](https://cdn.rupress.org/rup/content_public/journal/jem/214/12/10.1084_jem.20160783/5/jem_20160783_fig1.jpeg?Expires=1767986234&Signature=DjSFggl3IHNDTKOkVOpFbwyp46V1ImFOGj7pXGyhs1~pUGtkCV~tvntlLtdIv9JAETcbD0~JhpNvo-VX~Lc8mkcdH5UWXHFQMdh8DlVWveSw8XOjWumeZ9KCjmS4eSnmIQAtuGp2EMCMU~eFj9uqpgOqpajJJRGaiqNC19jX2zQs1JXGlBggWpD-wzhcEvkoFfTbmh7dNUfhudfDF~31SGrneQCTpe~N08vpGqh-QDe3ynwCOS9476qOuJ5hWURHULbivXztJ9Kb019JOTzuLbQEDv07MFlTtWmeJb~L6Uo29UO~X7SsCOKcJJhdEAoc3FdmV7ubW~GzrERa3T0lMw__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Image analysis workflow. (A) Overview of image analysis. (B) Preprocessing workflow. (C) Segmentation workflow. (D) Using the collagen signal (second harmonic generation) for classification between autofluorescence artifacts and GFP+RFP+MCs. Red channel refers to MCs, green channel refers to DCs, blue channel shows blood vessels, and gray channel shows collagen. Bars: (A–C and D [right]) 20 µm; (D, left) 50 µm. See also Fig. S1.
