Enhanced bacterial dissemination but protection from LPS-induced septic shock in Lyst-mutant Bg-J mice. (A) Dissemination of S. Typhimurium in orally infected WT and Bg-J mice was detected on day 4 after infection by determining CFU in spleen and liver. Data shown are combined results from three independent experiments. (B) Bronchoalveolar cell infiltration 24 h after intranasal inoculation of WT and Bg-J mice with LPS from S. Typhimurium or vehicle control. Data are pooled from three independent experiments. (C–G) WT and Bg-J mice were injected i.p. with LPS from S. Typhimurium (C and D) or with poly(I:C) (E–G). Blood was taken 1 h after LPS injection and 4 h after poly(I:C) injection. Serum concentrations of TNF (C and E), IFN-β (D and G), and IL-6 (F) were determined by ELISA. (H and I) WT and Bg-J mice were injected i.p. with Pam3CSK4. Blood was taken 2 h after injection, and serum concentrations of TNF (H) and IL-6 (I) were determined by ELISA. (C–I) Data are representative of at least two independent experiments. (A–I) Circles indicate values for individual animals (n ≥ 4), and bars indicate means. *, P < 0.05; **, P < 0.005 (Mann-Whitney U test). (J) Kaplan-Meier analysis of the survival of WT and Bg-J mice injected i.p. with LPS from S. Typhimurium. Mice were monitored over 7 d (n = 11 per genotype). ***, P < 0.0001 (log-rank test). Data are pooled from three independent experiments.