Figure 4. Testing potential in BM... | Rockefeller University Press

$Figure 4. Testing potential in BM subfractions responsible for radioprotection and early hematopoietic reconstitution after transplantation. (A) The experimental protocol to determine which BM subfractions are responsible for early hematopoiesis of granulocytes, erythrocytes, and megakaryocytes after transplantation. Indicated fractions obtained from KuO-Tg- or WT-B6 (Ly5.1) BM are cotransplanted competitively with WT-B6 (Ly5.1/5.2) mice KSL cells into lethally irradiated B6 (Ly5.2) recipients. (B) Percentages of KuO+ cell contribution in granulocytes, platelets, and erythrocytes (RBC) in recipient peripheral blood 2 wk after transplantation. Mean values are indicated as bars. n = 5 in each group. (C, left) A Kaplan-Meier plot showing survival among mice transplanted with indicated grafts. Log-rank testing was used to analyze survival data. P < 0.0001; P < 0.0125, Bonferroni-corrected threshold, was considered statistically significant. *, P < indicated Bonferroni-corrected threshold. (right) Percentages of B6 (Ly5.1) donor cell contribution in granulocytes in recipient peripheral blood 2 wk after transplantation. Mean values are indicated as bars. n = 7 in each group (only one mouse was alive and available at day 14 each for G1, G4, and G6, because of insufficient radioprotection). Representative data are shown from at least two independent experiments for each panel.$

Figure 4.

Testing potential in BM subfractions responsible for radioprotection and early hematopoietic reconstitution after transplantation. (A) The experimental protocol to determine which BM subfractions are responsible for early hematopoiesis of granulocytes, erythrocytes, and megakaryocytes after transplantation. Indicated fractions obtained from KuO-Tg- or WT-B6 (Ly5.1) BM are cotransplanted competitively with WT-B6 (Ly5.1/5.2) mice KSL cells into lethally irradiated B6 (Ly5.2) recipients. (B) Percentages of KuO⁺ cell contribution in granulocytes, platelets, and erythrocytes (RBC) in recipient peripheral blood 2 wk after transplantation. Mean values are indicated as bars. n = 5 in each group. (C, left) A Kaplan-Meier plot showing survival among mice transplanted with indicated grafts. Log-rank testing was used to analyze survival data. P < 0.0001; P < 0.0125, Bonferroni-corrected threshold, was considered statistically significant. *, P < indicated Bonferroni-corrected threshold. (right) Percentages of B6 (Ly5.1) donor cell contribution in granulocytes in recipient peripheral blood 2 wk after transplantation. Mean values are indicated as bars. n = 7 in each group (only one mouse was alive and available at day 14 each for G1, G4, and G6, because of insufficient radioprotection). Representative data are shown from at least two independent experiments for each panel.

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