Mapping interacting domains in TRIM65 and MDA5. (A) Immunoprecipitation (IP) and immunoblot analysis of the interaction of mCherry-MDA5 with Flag-tagged full-length TRIM65 protein or TRIM65 protein without the indicated domains in the lysates of HEK-293T cells. EV, empty vector. (B) IP and immunoblot analysis of the interaction of GFP-TRIM65 with full-length or truncated MDA5 in the lysates of HEK-293T cells. Data are representative of three independent experiments.