Figure 4.

Testing polyreactivity of IGHV4-34 antibodies. (A) Flow cytometric evaluation of binding preimmune and mutated IGHV4-34 and negative and low-positive control antibodies mGO53 and eiJB40, respectively, to intact (extracellular) and permeabilized (intracellular) HEK293 cells. Data are representative of two independent experiments. (B) Binding of IGHV4-34 IgG and control antibodies to LPS and dsDNA by ELISA. Data points are the mean and standard deviation of triplicate data points, representative of two independent experiments.

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