Comparison of TCRα and TCRβ repertoire among ABabDII mice, ABabDR4 mice, and human donors. (A) Absolute numbers of unique TCR amino acid clonotypes within 2.5 × 105 mouse or 1.8 × 105 human CD4 T cells. (B) Distribution of TCR amino acid clonotypes of different sizes: rare, 0 < x ≤ 0.001%; small, 0.001% < x ≤ 0.01%; mediumm 0.01% < x ≤ 0.1%; large, 0.1% < x < 1%; hyperexpanded (hyperexpan.), 1% < x < 10%. Human donor 3 was omitted from this analysis. Mann–Whitney test was used to compare clonotype sizes between ABabDII and ABabDR4 mice. (C) Percentage of medium to hyperexpanded TCR amino acid clonotypes of the total clonotypes. (D) Gini index indicating the equality of distribution of TCRα and TCRβ clonotypes (inequality). (E) Numbers of unique TCRα and TCRβ calculated using the iCHAO1 estimator. Data are from ABabDII (n = 5); ABabDR4 (n = 5) mice, and humans (n = 2 for B; n = 3 for A, C, and D). Mann–Whitney test (two-tailed) was used to compare values between ABabDII and ABabDR4 mice. **, 0.001 ≤ P < 0.05; *, 0.05 ≤ P < 0.1; n.s., not significant (Mann–Whitney test). Data from ABabDII and ABabDR4 mice are shown as means ± SD. Means and SD for humans are not depicted. In addition, Table 1 provides a summary of sequencing data and Fig. S2 provides further Treg and Tcon analysis.
