EAE recruits a phenotypically nonstimulatory population of CD11b⁺ myeloid cells to the lungs that is also retained in comorbid mice. (A–D) C57BL/6 mice were immunized and then inoculated as described in Fig. 1. (A) At 3 d (acute infection phase) and 11–16 d (influenza resolution phase) after influenza infection, whole lungs were perfused and harvested, and cellular composition was analyzed. Shown are representative flow cytometric plots of live-gated CD11b^hi Ly-6C^lo MDSCs and CD11b^lo Ly-6C^hi inflammatory monocytes (IMs). Compiled cellular percentages of inflammatory monocytes in both phases (B) and MDSCs in acute infection (C) and resolution phase (D) are shown from two to four independent experiments, with at least three mice per group. (E) Stimulatory phenotypes of inflammatory monocytes and MDSCs were also determined from mice in acute influenza infection phase. Shown are representative flow cytometric and histogram plots of live-gated cells and compiled cellular percentages of two to four independent experiments with at least three mice per group. MFI, mean fluorescence intensity. Student’s t tests were conducted for statistical analysis. *, P < 0.05; **, P < 0.01; ***, P < 0.001. The mean and standard error of means are represented.