EAE predisposes mice to increased morbidity and mortality from influenza infection. (A–D) C57BL/6 mice were immunized with MOG_35–55 EAE (A and B) or OVA_323–339 + CFA/PTX (C and D) on day 0. On day 7, naive mice and a subset of EAE or OVA-administered mice were inoculated with a sublethal dose of influenza. Mice were monitored daily for survival (A and C) and body weight loss (B and D). (B) Comparison of comorbid mouse body weight loss measurement to EAE and Flu mouse groups was ceased at day 22 because of significant loss of comorbid mice for meaningful analysis, denoted by the symbol #. Each graph is a combination of two independent experiments. (A and B) EAE, n = 7; Flu, n = 13; EAE + Flu, n = 20. (C and D) OVA + CFA/PTX, n = 8; Flu, n = 10; OVA + CFA/PTX + Flu, n = 16. To evaluate the significance of observed differences, the log-rank (Mantel-Cox) test (A and C) and one-way ANOVA (Tukey’s multiple comparison test; B and D) were used. **, P < 0.01; ***, P < 0.001. The mean and standard error of means are represented.