γδ T cells in LAT mutation. (A) FACS plots of γδ and αβ T cells in patient 2 and in a representative healthy control (top) and distribution of Vδ1 versus Vδ2 expression gated on γδ T cells (bottom). (B) Ca²⁺ mobilization in γδ T cells in the patient and a control. The arrow indicates the addition of goat anti-mouse for CD3 cross-linking. The experiment was performed once. (C) Percentage of spontaneous IL-4 producer (IL-4pos) and IFN-γ–positive (IFN-γpos) γδ T cells after PMA/ionomycin stimulation in two independent experiments compared with three healthy controls each. ctrl, control.