Figure 2. Surface MHC II turnover is reduced in the absence of MARCH E3 ligases. (A and B) Internalization of surface MHC II was measured for spleen DCs (A) and TECs (B). Cells were labeled with FIP-conjugated anti–MHC II antibody at 4°C (time 0). After 30 (A) or 60 (B) min of culture at 37°C, cells were removed and exposed to quencher (Q). The percentage of internalization is calculated as the FIP signal in the presence of Q (minus the signal elicited at time 0 with Q) as a percentage of the FIP signal at time 0 in the absence of Q (minus the signal elicited at time 0 with Q). Data are representative of five independent experiments (A) and two independent experiments (B) performed in triplicate. Bars represent the mean. *, P < 0.05; ***, P < 0.0001, unpaired Student’s t test.
Figure 2.

Surface MHC II turnover is reduced in the absence of MARCH E3 ligases. (A and B) Internalization of surface MHC II was measured for spleen DCs (A) and TECs (B). Cells were labeled with FIP-conjugated anti–MHC II antibody at 4°C (time 0). After 30 (A) or 60 (B) min of culture at 37°C, cells were removed and exposed to quencher (Q). The percentage of internalization is calculated as the FIP signal in the presence of Q (minus the signal elicited at time 0 with Q) as a percentage of the FIP signal at time 0 in the absence of Q (minus the signal elicited at time 0 with Q). Data are representative of five independent experiments (A) and two independent experiments (B) performed in triplicate. Bars represent the mean. *, P < 0.05; ***, P < 0.0001, unpaired Student’s t test.

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