Ets1 is required for mLN but not lung ILC2s. (A and B) FACS analysis for (A) and number of (B) Lin⁻KLRG1⁺SCA1⁺IL1RL1⁺ICOS⁺ ILC2s in the mLNs of LMC and Ets1^Δ/Δ mice. (C and D) FACS analysis for (C) and number of (D) ILC2s in the lungs of LMC and Ets1^Δ/Δ mice. (E) FACS analysis showing the contributions of RLMC or REts1^Δ/Δ (CD45.2) BM cells to the ILC2 population in the mLNs and lungs of mixed BM chimeras. ILC2s were identified using the staining strategies shown in A and C. For B and D, each symbol is one mouse and each bar is the mean. Error bars are SD. *, P < 0.05.