rag2^E450fsmutant zebrafish have reduced T cells and expanded myeloid and nkl.4⁺ cells. (A) Principal component analysis showing loss of T cells from the WKM of rag2^E450fs mutant zebrafish and expansion of nkl.4⁺ cells (black). WT and rag2^E450fs WKM were included in PCA. For visualization, cells from rag2^E450fs WKM are shown in color, and WT WKM cells are greyed circles. (B) Quantification of blood cell types from the marrow of WT and rag2^E450fs mutant zebrafish. Counts were based on single-cell PCR gene expression and assignments based on hierarchical clustering defined in Fig. 3. *, P = 0.0081; **, P = 0.0001, by Fisher’s exact test. The numbers of cells per lineage are noted within the bar graph. n = 166 WT WKM cells and n = 112 rag2^E450fs WKM cells combined from two animals. (C) Violin plots show the distribution of gene expression in nkl.4⁺ cells. (D) Genes expressed within each cell type.