Identification of CD41:GFP^low hematopoietic stem and precursor cells. (A) Unsupervised hierarchical clustering comparing gene expression of unlabeled WKM and Tg(CD41:GFP)^low cells. Six major lineages were identified including erythroid (red), myeloid (green), B cells (light blue), T cells (dark blue), myeloid-associated CD41:GFP^low cells (denoted by light purple shading in dendogram), and HSPCs (purple). The cell identity of each sorted cell is shown as rectangles immediately below the dendograms, unlabeled WKM (black), and Tg(CD41:GFP)^low (purple). n = 166 WT WKM cells (combined from two animals) and n = 85 CD41:GFP^low WKM cells from a single animal. (B) Pie charts showing proportion of blood lineages in Tg(CD41:GFP)^low cells when compared with unlabeled WKM cells. (C) Principal component analysis of unlabeled WKM and Tg(CD41:GFP)^low cells. (D) Violin plots show the distribution of gene expression of single cells. Cells types were assigned based on hierarchical clustering and assessed for transcript expression of well-known blood cell lineage genes.