Figure 3. Unlabeled marrow cells cluster efficiently with single cells isolated from transgenic reporter lines. (A) Principal component analysis identifies four major blood lineages in WKM. Filled circles are unlabeled WKM, open circles are peripheral red blood cells. Cells isolated from transgenic reporter lines (gata1:dsRed, red; mpx:GFP, green; rag2:dsRed, light blue; or lck:GFP, dark blue) are denoted by an x. (B) Blood cells from transgenic fish reside within expected cell lineages as defined by hierarchical clustering with unlabeled marrow. (C) Violin plots confirm that cells assigned to specific hierarchical clusters have high expression of cell lineage–restricted genes. Unlabeled peripheral RBCs (per RBC) and marrow cells (erythroid, myeloid, B cells, and T cells). Sorted transgenic cells denoted by promoter.
Figure 3.

Unlabeled marrow cells cluster efficiently with single cells isolated from transgenic reporter lines. (A) Principal component analysis identifies four major blood lineages in WKM. Filled circles are unlabeled WKM, open circles are peripheral red blood cells. Cells isolated from transgenic reporter lines (gata1:dsRed, red; mpx:GFP, green; rag2:dsRed, light blue; or lck:GFP, dark blue) are denoted by an x. (B) Blood cells from transgenic fish reside within expected cell lineages as defined by hierarchical clustering with unlabeled marrow. (C) Violin plots confirm that cells assigned to specific hierarchical clusters have high expression of cell lineage–restricted genes. Unlabeled peripheral RBCs (per RBC) and marrow cells (erythroid, myeloid, B cells, and T cells). Sorted transgenic cells denoted by promoter.

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