Figure 2. Single cells isolated from lineage-restricted transgenic reporter lines segregate together after hierarchical clustering. (A) FACS analysis (left) and cytospins of sorted transgenic blood populations (right). Bars, 25 µm. (B) Unsupervised hierarchical clustering after single-cell gene expression analysis. Cell identity is shown above the heat map: peripheral blood cells (pink, n = 20 cells) and sorted fluorescent WKM cells from gata1:dsRed (red, n = 24 cells), mpx:GFP (green, n = 48 cells), rag2:dsRed (light blue, n = 49 cells), or lck:GFP (dark blue, n = 83 cells). Cells shown are from a single representative animal. Yellow shading denotes cells that failed to cluster into known lineages described by our qPCR panel.
Figure 2.

Single cells isolated from lineage-restricted transgenic reporter lines segregate together after hierarchical clustering. (A) FACS analysis (left) and cytospins of sorted transgenic blood populations (right). Bars, 25 µm. (B) Unsupervised hierarchical clustering after single-cell gene expression analysis. Cell identity is shown above the heat map: peripheral blood cells (pink, n = 20 cells) and sorted fluorescent WKM cells from gata1:dsRed (red, n = 24 cells), mpx:GFP (green, n = 48 cells), rag2:dsRed (light blue, n = 49 cells), or lck:GFP (dark blue, n = 83 cells). Cells shown are from a single representative animal. Yellow shading denotes cells that failed to cluster into known lineages described by our qPCR panel.

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