CLR/CARD9 cascade is required for host Cbl-b–mediated antifungal immune regulation. (A) Survival of WT, Cbl-b KO, Dectin-3 KO, or Cbl-b/Dectin-3 dKO mice (n = 10 per group) infected intravenously with 105 CFU of C. albicans (SC5314). (B) Kidney CFU assay of WT, Cbl-b KO, Dectin-3 KO, or Cbl-b/Dectin-3 dKO mice (n = 15 per group) infected intravenously with 105 CFU of C. albicans (SC5314) for 24 h. The data shown are representative of three independent and reproducible experiments. (C) Amounts of TNF and IL-6 in kidneys of WT, Cbl-b KO, Dectin-3 KO, or Cbl-b/Dectin-3 dKO mice after infection with 105 CFU of C. albicans (SC5314) for 24 h. Data are means ± SD of triplicate samples and are representative of three independent experiments. (D) Survival of WT, Cbl-b KO, CARD9 KO, or Cbl-b/CARD9 dKO mice (n = 10 per group) infected intravenously with 105 CFU of C. albicans (SC5314). (E) Kidney CFU assay of WT, Cbl-b KO, CARD9 KO, or Cbl-b/CARD9 dKO mice (n = 5 per group) after intravenous infection with 105 CFU of C. albicans (SC5314) for 24 h. The data shown are representative of three independent and reproducible experiments. (F) Amounts of TNF, IL-6, IL-1β, and IL-12p40 in kidneys of WT, Cbl-b KO, CARD9 KO, or Cbl-b/CARD9 dKO mice after infection with 105 CFU of C. albicans (SC5314) for 24 h. Data are means ± SD of triplicate samples and are representative of three independent experiments. *, P < 0.05; **, P < 0.01; ***, P < 0.001 (ANOVA).
