Cbl-b negatively regulates in vivo CLR-mediated antifungal immunity. (A) Survival of WT and Cbl-b KO mice (n = 10 per group) infected intravenously with 105 CFU of C. albicans (SC5314). (B) Kidney CFU assay of WT and Cbl-b KO mice (n = 5 per group) infected intravenously with 105 CFU of C. albicans (SC5314) for 24 h. (C) Amounts of TNF and IL-6 in kidneys of infected WT and Cbl-b KO mice after infection with 105 CFU of C. albicans (SC5314) for 24 h. (D) Quantitative real-time PCR assay of chemokines including CXCL1, CXCL2, and CCL3 in kidneys of WT and Cbl-b KO mice after infection with 105 CFU of C. albicans (SC5314) for 24 h. (E) Lung CFU assay of WT and Cbl-b KO mice (n = 3 per group) after trans-nasal infection with 106 CFU of A. fumigatus conidia for 24 h. The data shown are representative of three independent and reproducible experiments. (F) Amounts of TNF and IL-6 in lungs of WT and Cbl-b KO mice after trans-nasal infection with 106 CFU of A. fumigatus conidia for 24 h. (C, D, and F) Data are means ± SD of triplicate samples and are representative of three independent experiments. **, P < 0.01; ***, P < 0.001 (Student’s t test).
