Reactivity to nontargeted epitopes. Heat map summarizing responses of CD8⁺ and CD4⁺ T cells independent of HLA restriction to pools of 20–30 peptides spanning MART1 (red), NY-ESO1 (blue), gp100 (green), tyrosinase (violet), and MAGE-A3 (orange). The color scale (light to dark) reflects the response magnitude at indicated time points before and after administration of monoclonal and polyclonal CTL during the patients’ treatment course (top schema). Inset numbers indicate IFN-γ spots per 10⁵ PBMC for each peptide pool.